[1]Department of Pediatric Surgery, Qilu Hospital of Shandong University, Jinan, China. Electronic address: 202236137@mail.sdu.edu.cn.
[2]Department of Pediatric Surgery, Qilu Hospital of Shandong University, Jinan, China. Electronic address: liguowei@qiluhospital.com.
[3]Department of Pediatrics, Qilu Hospital of Shandong University, Jinan, China. Electronic address: liyingying@qiluhospital.com.
[4]Department of Pediatrics, Qilu Hospital of Shandong University, Jinan, China. Electronic address: gaohongjie@qiluhospital.com.
[5]Department of Pediatric Surgery, Qilu Hospital of Shandong University, Jinan, China. Electronic address: sunfengyin@qiluhospital.com.
Lactic acid (LA) can promote the malignant progression of tumors through the crosstalk with the tumor microenvironment (TME). However, the function of long non-coding RNAs (lncRNAs) related to LA metabolism in Wilms tumor (WT) remains unclear.;Gene expression data and clinical data of WT patients were collected from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO). Through the ESTIMATE algorithm and Pearson correlation analysis, lncRNAs related to tumor immunity and LA metabolism were screened. Subsequently, Cox regression analysis and Lasso Cox regression analysis were used to construct a model. Furthermore, candidate genes were identified and a competitive endogenous RNA (ceRNA) network was conducted to explore the specific mechanism of characteristic genes. Finally, based on the strong clinical relevance of UNC5B-AS1, its expression and function were experimentally verified.;The immune score and stromal score were found to be closely related to the prognosis of WT. Eventually, a prognostic model (TME-LA-LM) consisting of 6 lncRNAs was successfully identified. The model demonstrated favorable predictive ability and accuracy, with significant variation in immune infiltration and drug susceptibility observed between risk groups. Additionally, the study revealed the involvement of 2 candidate genes and 5 microRNAs (miRNAs) in the tumor's development. Notably, UNC5B-AS1 was highly expressed and found to promote the proliferation and migration of tumor cells.;This study, for the first time, elucidated the prognostic signatures of WT using lncRNAs related to TME and LA metabolism. The fundings of this research offer valuable insights for future studies on immunotherapy, personalized chemotherapy and mechanism research.;
(8.0+极速模式)